HyCyte cell line: HyCyte L-02 stable cell line for hPCSK9 overexpression

Catalogue No.: CGSC-M32581

Gene: hPCSK9

Gene ID: 255738

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：800

Target transcript: NM_174936.4

Modification type: Poolcells

Fluorescent gene: EGFP&mCherry

Resistance gene: Neo

Parental：L-02

Species：Human

Passage：5

Doubling time：24-36h

Growth properties: Adherent

Recommended culture media: RPMI-1640 supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G446).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:4-1:8.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).