HyCyte cell line: HyCyte A-549 stable cell line for hUPP1 overexpression

Catalogue No.: CGSC-M32401

Gene: hUPP1

Gene ID: 7378

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：1

Target   transcript: NM_003364.4，933 bp

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：A-549

Species：Human

Passage：5

Doubling time：36-48h

Growth properties: Adherent

Recommended culture media: Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (D/F12) supplemented with 10 % FBS,100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G116).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: D/F12 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).