HyCyte cell line: HyCyte C666-1 stable cell line for hWDR54 overexpression

Catalogue No.: CGSC-M32391

Gene:hWDR54

Gene ID: 84058

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：1

Target transcript: NM_001320823.2

ModificationType: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：C666-1

Species：Human

Passage：5

Doubling time：24--36h

Growth properties: Adherent

Recommended culture media: RPMI-1640supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G439)

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).