HyCyte cell line:HyCyte Ba/F3 stable cell line for mPtk2 overexpression

Catalogue No.: CGSC-M32291

Gene:mPtk2

Gene ID: 14083

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：1

Target transcript: NM_001358045.1

ModificationType: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：Ba/F3

Species：Mouse

Passage：5

Doubling time：18--24h

Growth properties: Suspension

Recommended culture media: RPMI-1640 supplemented with 10 % FBS, 10 ng/mL mouse IL-3, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCM-G786)

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. The cell culture density is 3×10⁵ to 5×10⁵ cells per mL.

Freezing media:RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).