HyCyte cell line: HyCyte THP-1 stable cell line for hPDHA1 overexpression

Catalogue No.: CGSC-M32271

Gene: hPDHA1

Gene ID: 5160

Transduction/transfection methods: Lentivirus

Resistance concentration (μg/mL)：1

Target transcript: NM_001173454.2

Modification type: Poolcells

Fluorescent gene: ——

Resistance gene: Puro

Parental：THP-1

Species：Human

Passage：5

Doubling time：24-36h

Growth properties: Suspension

Recommended culture media: RPMI-1640 supplemented with 10% FBS, 0.05 mM β-mercaptoethanol, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G361).

Subculture: Cells are cultured as a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. The cell culture density is 5×10⁵ to 1×10⁶ cells per mL.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).