HyCyte cell line: HyCyte SH-SY5Y stable cell line for hGDF15 overexpression

Catalogue No.: CGSC-M32261

Gene: hGDF15

Gene ID: 9518

Transduction/transfection methods: Lentivirus

Resistance concentration (μg/mL)：1

Target transcript: NM_004864.4

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：SH-SY5Y

Species：Human

Passage：5

Doubling time：48-60 h

Growth Properties: Semi-adherent

Recommended culture media: Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (D/F12) supplemented with 15 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G325).

Subculture: Cells are cultured as a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.

Freezing media: D/F12 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).