HyCyte cell line: HyCyte HCT 116 stable cell line for hTRIM28 overexpression

Catalogue No.: CGSC-M32171

Gene: hTRIM28

Gene ID: 10155

Transduction/transfection methods: Transposon

Resistance concentration(μg/mL)：1000

Target transcript: NM_005762.3

Modification type: Poolcells

Fluorescent gene: EYFP

Resistance gene: Puro

Parental：HCT116

Species：Human

Passage：5

Doubling time：24-36h

Growth properties: Adherent

Recommended culture media: McCoy's 5A Medium supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin(HyCyte,TCH-G185).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:8.

Freezing media: McCoy's 5A + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201)