HyCyte cell line: HyCyte U-937 stable cell line for hHIF1A overexpression

Catalogue No.: CGSC-M31861

Gene: hHIF1A

Gene ID: 3091

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：0.8

Target transcript: NM_001530.4

Modification type: Poolcells

Fluorescent gene: mCherry

Resistance gene: Puro

Parental：U-937

Species：Human

Passage：5

Doubling time：95-100h

Growth properties: Suspension

Recommended culture media: RPMI-1640 supplemented with 10% FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin(HyCyte,TCH-G368).

Subculture: Cells are cultured as a monolayer at 37℃ in a humidified atmosphere with 5%CO₂. Cells should be passaged every 2-3 days. The cell culture density is 3×10⁵ to 5×10⁵ cells per mL.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).