HyCyte cell line: HyCyte 5637 stable cell line for hFGFR3 overexpression

Catalogue No.: CGSC-M31811

Gene:hFGFR3

Gene ID:2261

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：0.3

Target transcript:NM_001163213.2

ModificationType: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：5637

Species：Human

Passage：5

Doubling time：24--36h

Growth properties: Adherent

Recommended culture media:

RPMI-1640 supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G104).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).

Genotype: hCKAP4 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative