HyCyte cell line: HyCyte SW620 stable cell line for hEPAS1 overexpression

Catalogue No.: CGSC-M31731

Gene: hEPAS1

Gene ID: 2034

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：2

Target transcript: NM_001430.5

ModificationType: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：SW620

Species：Human

Passage：5

Doubling time：36--48h

Growth properties: Adherent

Recommended culture media:

Leibovitz L-15 Medium supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G350).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 0% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:3.

Freezing media: L-15+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201)

Genotype: hHKDC1 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative