HyCyte cell line: HyCyte HT22 stable cell line for hTFEB overexpression

Catalogue No.: CGSC-M31281

Gene: hTFEB

Gene ID: 7942

Transduction/transfection methods: Transposon

Resistance concentration(μg/mL)：8

Target transcript: NM_007162.3

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：HT22

Species：Mouse

Passage：5

Doubling time：24-36h

Growth properties: Adherent

Recommended culture media: Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCM-G821).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:4-1:6.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).