HyCyte cell line: HyCyte GES-1 stable cell line for hGBP5 overexpression

Catalogue No.: CGSC-M31271

Gene: hGBP5

Gene ID: 115362

Transduction/transfection methods: Transposon

Resistance concentration(μg/mL)：1

Target transcript: NM_052942.5

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：GES-1

Species：Human

Passage：5

Doubling time：18-24h

Growth properties: Adherent

Recommended culture media: RPMI-1640 supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin(HyCyte,TCH-G410).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).