HyCyte cell line: HyCyte RenCa stable cell line for mScarb1 overexpression

Catalogue No.: CGSC-M31261

Gene: mScarb1

Gene ID: 20778

Transduction/transfection methods: Lentivirus

Resistance concentration (μg/mL)：0.5

Target transcript: NM_001205083.2

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：RenCa

Species：Mouse

Passage：5

Doubling time：24-36h

Growth properties: Adherent

Recommended culture media: RPMI-1640 supplemented with 10% FBS, 2 mM Glutamax, 1 mM Sodium Pyruvate,1% NEAA,100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCM-G784).

Subculture: Cells are cultured as a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:5.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).