HyCyte cell line: HyCyte Caov-3 stable cell line for hROR1 overexpression

Catalogue No.: CGSC-M31191

Gene: hROR1

Gene ID: 4919

Transduction/transfection methods: Transposon

Resistance concentration(μg/mL)：0.5

Target transcript: NM_005012.4

Modification type: Poolcells

Fluorescent gene: luciferase

Resistance gene: Puro

Parental：Caov-3

Species：Human

Passage：5

Doubling time：72-84h

Growth properties: Adherent

Recommended culture media: Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G593).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).