HyCyte cell line: HyCyte EMT6 stable cell line for hNECTIN4 overexpression

Catalogue No.: CGSC-M31181

Gene: hNECTIN4

Gene ID: 81607

Transduction/transfection methods: Transposon

Resistance concentration(μg/mL)：2

Target transcript: NM_030916.3

Modification type: Single Clone

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：EMT6

Species：Mouse

Passage：5

Doubling time：24-48h

Growth properties: Adherent

Recommended culture media: RPMI-1640 supplemented with 15 % FBS, 2mM Glutamax, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCM-G728).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:4-1:8.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).