HyCyte cell line: HyCyte MC-38 stable cell line for mSlc5a5 overexpression

Catalogue No.: CGSC-M31161

Gene: mSlc5a5

Gene ID: 114479

Transduction/transfection methods: Lentivirus

Resistance concentration(μg/mL)：6

Target transcript: NM_053248.2

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：MC-38

Species：Mouse

Passage：5

Doubling time：18-24h

Growth properties: Adherent

Recommended culture media: RPMI-1640 supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin(HyCyte,TCM-G790).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).