HyCyte cell line: HyCyte RAW 264.7 stable cell line for mMettl3 overexpression

Catalogue No.: CGSC-M31071

Gene: mMettl3

Gene ID: 56335

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：8

Target transcript: NM_019721.2

Modification type: Poolcells

Fluorescentgene: ——

Resistancegene: Puro

Parental：RAW 264.7

Species：Mouse

Passage：5

Doubling time：18-24h

Growth properties: Adherent

Recommended culture media: Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10%FBS, 2 mM Glutamax, 1 mM Sodium Pyruvate,100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCM-G766).

Subculture: Cells are cultured as a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).