HyCyte cell line: HyCyte PC-9 stable cell line for hIL6 overexpression

Catalogue No.: CGSC-M30981

Gene: hIL6

Gene ID: 3569

Transduction/transfection methods: Transposon

Resistance concentration(μg/mL)：4

Target transcript: NM_000600.5

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：PC-9

Species：Human

Passage：5

Doubling time：24-32h

Growth properties: Adherent

Recommended culture media: RPMI-1640 supplemented with 10% FBS, 2 mM Glutamax, 1 mM Sodium Pyruvate,100 U/mL Penicilinand 100 µg/mL Streptomycin (HyCyte,TCH-G380).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:4-1:6.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).