HyCyte cell line: HyCyte IEC-6 stable cell line for rAcsf2 overexpression

Catalogue No.: CGSC-M30941

Gene: rAcsf2

Gene ID: 619561

Transduction/transfection methods: Transposon

Resistance concentration(μg/mL)：4

Target transcript: NM_001034951.3

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：IEC-6

Species：Rat

Passage：5

Doubling time：24-36h

Growth properties: Adherent

Recommended culture media: Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10% FBS, 10 µg/mL Human recombinant insulin, 100 U/mL Penicilin and 100 µg/mL Streptomycin(HyCyte,TCR-G626).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media: DMEM + 30% FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).