HyCyte cell line: HyCyte HK-2 [Humankidney] stable cell line for hAPELA overexpression

Catalogue No.: CGSC-M30871

Gene: hAPELA

Gene ID: 100506013

Transduction/transfection methods: Transposon

Resistance concentration(μg/mL)：2

Target transcript: NM_001297550.2

Modification type: Poolcells

Fluorescent gene: ——

Resistance gene: Puro

Parental：HK-2[Human kidney]

Species：Human

Passage：5

Doubling time：24-36h

Growth properties: Adherent

Recommended culture media: Minimum EssentialMedium (MEM) supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G400).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.

Freezing media: MEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).