HyCyte cell line: HyCyte H9c2(2-1) stable cell line for rKeap1 overexpression

Catalogue No.: CGSC-M30821

Gene: rKeap1

Gene ID: 117519

Transduction/transfection methods: Transposon

Resistance concentration(μg/mL)：4

Target transcript: NM_057152.2

Modification type: Poolcells

Fluorescent gene: EYFP

Resistance gene: Puro

Parental：H9c2(2-1)

Species：Rat

Passage：5

Doubling time：48-72h

Growth properties: Adherent

Recommended culture media: Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 2 mM Glutamax,100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCR-G607).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).