HyCyte cell line: HyCyte MHCC97-H stable cell line for hPLAUR overexpression

Catalogue No.: CGSC-M30651

Gene: hPLAUR

Gene ID: 5329

Transduction/transfection methods: Transposon

Resistance concentration(μg/mL)：4

Target transcript: NM_002659.4

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：MHCC97-H

Species：Human

Passage：5

Doubling time：60-72h

Growth properties: Adherent

Recommended culture media: Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 2 mM Glutamax,1 mM Sodium Pyruvate,100 U/mL Penicilin and 100 µg/mL Streptomycin(HyCyte,TCH-G258).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).