HyCyte cell line: HyCyte LL/2 (LLC1)stable cell line for mBnip3 overexpression

Catalogue No.: CGSC-M30421

Gene: mBnip3

Gene ID: 12176

Transduction/transfection methods: Transposon

Resistance concentration(μg/mL)：4

Target transcript: NM_009760.4

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：LL/2(LLC1)

Species：Mouse

Passage：5

Doubling time：18-24h

Growth properties: Semi-adherent

Recommended culture media: Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCM-G742).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).