HyCyte cell line: HyCyte C8-D1A stable cell line for mMmp9 overexpression

Catalogue No.: CGSC-M30371

Gene: mMmp9

Gene ID: 17395

Transduction/transfection methods: Transposon

Resistance concentration(μg/mL)：2

Target transcript: NM_013599.5

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：C8-D1A

Species：Mouse

Passage：5

Doubling time：48-60h

Growth properties: Adherent

Recommended culture media: Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCM-G825).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:2.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).