HyCyte cell line: HyCyte MCF-7 stable cell line for hDDR2 overexpression

Catalogue No.: CGSC-M30181

Gene: hDDR2

Gene ID: 4921

Transduction/transfection methods: Lentivirus

Resistance concentration(μg/mL)：4

Target transcript: NM_006182.2

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：MCF-7

Species：Human

Passage：5

Doubling time：36-48h

Growth properties: Adherent

Recommended culture media: Minimum Essential Medium (MEM) supplemented with 10 % FBS, 2 mM Glutamax,1 mM Sodium Pyruvate,1% NEAA, 10 µg/mL Human recombinant insulin,100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G247).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: MEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).