HyCyte cell line: HyCyte Caco-2 stable cell line for hSERPINA4 overexpression

Catalogue No.: CGSC-M30071

Gene: hSERPINA4

Gene ID: 5267

Transduction/transfection methods: Transposon

Resistance concentration(μg/mL)：2

Target transcript: NM_006215.4

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：Caco-2

Species：Human

Passage：5

Doubling time：36-48h

Growth properties: Adherent

Recommended culture media: Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 20 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G146).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step FreezingMedium (HyCyte, GUCP-R201).