HyCyte cell line: HyCyte BRL-3Astable cell line for rPpargc1b overexpression

Catalogue No.: CGSC-M29981

Gene: rPpargc1b

Gene ID: 291567

Transduction/transfection methods: Transposon

Resistance concentration(μg/mL)：4

Target transcript: NM_176075.3

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：BRL-3A

Species：Rat

Passage：5

Doubling time：18-24h

Growth properties: Adherent

Recommended culture media: Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCR-G604).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).