HyCyte cell line: HyCyte Hep-G2 stable cell line for hSLC6A3 overexpression

Catalogue No.: CGSC-M29831

Gene: hSLC6A3

Gene ID: 6531

Transduction/transfection methods: Transposon

Resistance concentration(μg/mL)：2

Target transcript: NM_001044.5

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：Hep-G2

Species：Human

Passage：5

Doubling time：60-72h

Growth properties: Adherent

Recommended culture media: Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 2 mM Glutamax, 1 mM Sodium Pyruvate,100 U/mL Penicilin and 100 µg/mL Streptomycin(HyCyte,TCH-G196).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).