HyCyte cell line: HyCyte SU-DHL-4 stable cell line for hEBF1 overexpression

Catalogue No.: CGSC-M29441

Gene: hEBF1

Gene ID: 1879

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：0.5

Target transcript: NM_024007.5

Modification type: Poolcells

Fluorescent gene: luciferase

Resistance gene: Puro

Parental：SU-DHL-4

Species：Human

Passage：5

Doubling time：36-48h

Growth properties: Suspension

Recommended culture media: RPMI-1640 supplemented with 10% FBS, 2 mM Glutamax,1 mM Sodium Pyruvate,100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G428).

Subculture: Cells are cultured as a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. The cell culture density is 3×10⁵ to 5×10⁵ cells per mL.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).