HyCyte cell line: HyCyte SW1353 stable cell line for hHMGA2 overexpression

Catalogue No.: CGSC-M29321

Gene: hHMGA2

Gene ID: 8091

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：3

Target transcript: NM_003483.6

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：SW1353

Species：Human

Passage：5

Doubling time：24-48h

Growth properties: Adherent

Recommended culture media: Leibovitz L-15 Medium supplemented with 10% FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G342).

Subculture: Cells are cultured as a monolayer at 37℃ in a humidified atmosphere with 0% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media: L-15+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201)