HyCyte cell line: HyCyte EA.hy 926 stable cell line for hHIF1A overexpression

Catalogue No.: CGSC-M29251

Gene: hHIF1A

Gene ID: 3091

Transduction/transfection methods: Transposon

Resistance concentration(μg/mL)：1

Target transcript: NM_001243084.2

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：EA.hy926

Species：Human

Passage：5

Doubling time：48-60h

Growth properties: Adherent

Recommended culture media: Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G171).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).