HyCyte cell line: HyCyte BV-2 stablecell line for mDnm1 overexpression

Catalogue No.: CGSC-M29141

Gene: mDnm1

Gene ID: 13803

Transduction/transfection methods: Transposon

Resistance concentration(μg/mL)：2

Target transcript: NM_001360007.2

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：BV-2

Species：Mouse

Passage：5

Doubling time：18-24h

Growth properties: Semi-adherent

Recommended culture media: Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCM-G718).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).