HyCyte cell line: HyCyte HK-2 [Human kidney] stable cell line for hNCOA4 overexpression

Catalogue No.: CGSC-M28861

Gene: hNCOA4

Gene ID: 8031

Transduction/transfection methods: Lentivirus

Resistance concentration (μg/mL)：2

Target transcript: NM_005437.4

Modification type: Poolcells

Fluorescent gene: ——

Resistance gene: Puro

Parental：HK-2 [Human kidney]

Species：Human

Passage：5

Doubling time：24-36h

Growth properties: Adherent

Recommended culture media: Minimum Essential Medium (MEM) supplemented with 10% FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G400).

Subculture: Cells are cultured as a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.

Freezing media: MEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).