HyCyte cell line: HyCyte AC16 [Humanhybrid cardiomyocyte] stable cell line for hRCAN3 overexpression

Catalogue No.: CGSC-M28821

Gene: hRCAN3

Gene ID: 11123

Transduction/transfection methods: Lentivirus

Resistance concentration (μg/mL)：1

Target transcript: NM_013441.4

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：AC16[Human hybrid cardiomyocyte]

Species：Human

Passage：5

Doubling time：24-36h

Growth properties: Adherent

Recommended culture media: Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (D/F12) supplemented with 12.5 %FBS, 2mM Glutamax, 100 U/mL Penicilin and 100 µg/mL Streptomycin(HyCyte,TCH-G119).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: D/F12 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).