HyCyte cell line: HyCyte MKN45 stable cell line for hALPP overexpression

Catalogue No.: CGSC-M28511

Gene: hALPP

Gene ID: 250

Transduction/transfection methods: Transposon

Resistance concentration(μg/mL)：4

Target transcript: NM_001632.5

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：MKN45

Species：Human

Passage：5

Doubling time：36-48h

Growth properties: Semi-adherent

Recommended culture media: RPMI-1640 supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin(HyCyte,TCH-G397).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).