HyCyte cell line: HyCyte Jurkat stable cell line for hCD72 overexpression

Catalogue No.: CGSC-M28491

Gene: hCD72

Gene ID: 971

Transduction/transfection methods: Transposon

Resistance concentration(μg/mL)：0.5

Target transcript: NM_001782.3

Modification type: Single Clone

Fluorescent gene: ——

Resistance gene: Puro

Parental：Jurkat

Species：Human

Passage：5

Doubling time：36-48h

Growth properties: Suspension

Recommended culture media: RPMI-1640 supplemented with 10 % FBS, 2 mM Glutamax, 1 mM Sodium Pyruvate, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G225).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. The cell culture density is 5×10⁵ to 1×10⁶ cells per mL.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).