HyCyte cell line: HyCyte Panc02 stable cell line for mCxcr2 overexpression

Catalogue No.: CGSC-M28441

Gene: mCxcr2

Gene ID: 12765

Transduction/transfection methods: Transposon

Resistance concentration(μg/mL)：4

Target transcript: NM_009909.3

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：Panc02

Species：Mouse

Passage：5

Doubling time：36-48h

Growth properties: Adherent

Recommended culture media: RPMI-1640 supplemented with 10% FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin(HyCyte,TCM-G811).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).