HyCyte cell line: HyCyte SiHa stable cell line for hNUDT5 overexpression

Catalogue No.: CGSC-M27791

Gene: hNUDT5

Gene ID: 11164

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：1

Target transcript: NM_014142.4

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：SiHa

Species：Human

Passage：5

Doubling time：72-96h

Growth properties: Adherent

Recommended culture media: Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10%FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G326).

Subculture: Cells are cultured as a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).