HyCyte cell line: HyCyte INS-1 stable cell line for rAbcc8 overexpression

Catalogue No.: CGSC-M27721

Gene: rAbcc8

Gene ID: 25559

Transduction/transfection methods: Transposon

Resistance concentration(μg/mL)：1

Target transcript: NM_013039.3

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：INS-1

Species：Rat

Passage：5

Doubling time：96-120h

Growth properties: Adherent

Recommended culture media: RPMI-1640 supplemented with 10% FBS,0.05 mM β-mercaptoethanol, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCR-G608).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).