HyCyte cell line: HyCyte HL-60 stable cell line for hHSPA5 overexpression

Catalogue No.: CGSC-M27655

Gene: hHSPA5

Gene ID: 3309

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：0.5

Target transcript: NM_005347.5

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：HL-60

Species：Human

Passage：5

Doubling time：24-36h

Growth properties: Suspension

Recommended culture media: Iscove's Modified Dulbecco's Medium supplemented with 20 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G204).

Subculture: Cells are cultured as a monolayer at 37℃ in a humidified atmosphere with 5%CO₂. Cells should be passaged every 2-3 days. The cell culture density is 3×10⁵ to 5×10⁵ cells per mL.

Freezing media: IMDM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201)