HyCyte cell line: HyCyte HEK293T stable cell line for hABCB1 overexpression

Catalogue No.: CGSC-M27521

Gene: hABCB1

Gene ID: 5243

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：2.0

Target transcript: NM_001348945.2

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：HEK293T

Species：Human

Passage：5

Doubling time：24-48h

Growth Properties: Adherent

Recommended culture media: Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G101).

Subculture: Cells are cultured as a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:4-1:8.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).