HyCyte cell line: HyCyte U-87MG ATCC stable cell line for hAPOE overexpression

Catalogue No.: CGSC-M27221

Gene: hAPOE

Gene ID: 348

Transduction/transfection methods: Lentivirus

Resistance concentration (μg/mL)：1

Target transcript: NM_001302691.2

Modification type: SingleClone

Fluorescent gene: ——

Resistance gene: Puro

Parental：U-87MG ATCC

Species：Human

Passage：5

Doubling time：72-96h

Growth properties: Adherent

Recommended culture media: Minimum Essential Medium (MEM) supplemented with 10% FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G367).

Subculture: Cells are cultured as a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media: MEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).