HyCyte cell line: HyCyte TPC-1 stable cell line for hLCN2 overexpression

Catalogue No.: CGSC-M27171

Gene: hLCN2

Gene ID: 3934

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：1

Target transcript: NM_005564.5

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：TPC-1

Species：Human

Passage：5

Doubling time：18-24h

Growth properties: Adherent

Recommendedculture media: RPMI-1640 supplemented with 10% FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G381).

Subculture: Cells are cultured as a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:4-1:6.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).