HyCyte cell line: HyCyte HK-2 [Humankidney] stable cell line for hAQP1 overexpression

Catalogue No.: CGSC-M27121

Gene: hAQP1

Gene ID: 358

Transduction/transfection methods: Lentivirus

Resistance concentration(μg/mL)：2

Target transcript: NM_198098.4

Modification Type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：HK-2[Human kidney]

Species：Human

Passage：5

Doubling time：24-36h

Growth properties: Adherent

Recommended culture media: Minimum EssentialMedium (MEM) supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G400).

Subculture: Cells are culturedas a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.

Freezing media: MEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).