HyCyte cell line: HyCyte MIN6 stable cell line for mRpl22 overexpression

Catalogue No.: CGSC-M27021

Gene: mRpl22

Gene ID: 19934

Transduction/transfection methods: Transposon

Resistance concentration(μg/mL)：1

Target transcript: NM_001277113.1

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：MIN6

Species：Mouse

Passage：5

Doubling time：36-48h

Growth properties: Adherent

Recommended culture media: Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 15% FBS, 2 mM Glutamax, 0.05 mM β-mercaptoethanol, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCM-G787).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:3.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).