HyCyte cell line: HyCyte HeLa stable cell line for hEIF3A overexpression

Catalogue No.: CGSC-M26771

Gene: hEIF3A

Gene ID: 8661

Transduction/transfection methods: Transposon

Resistance concentration(μg/mL)：2

Target transcript: NM_024504.4

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：HeLa

Species：Human

Passage：5

Doubling time：30-48h

Growth properties: Adherent

Recommended culture media: Minimum EssentialMedium (MEM) supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G193).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:6.

Freezing media: MEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).