Cell Name: Stable-overexpressing mouse Dlk1 in RAW264.7 cell line
Catalogue No.: CGSC-M26641

Modified Gene: mDlk1

Gene ID: 13386   

Modification Type: Overexpression

Method: Piggy-BAC

Transcript: NM_010052.6

Resistance gene: Puro

Fluorescent gene: EGFP

Clone Type: Poolcells

Cellular Identification: PCR, Sequencing

Genus: Mus musculus

Tissue origin: Monocytes; macrophages

Cell morphology: Irregularly rounded

Characteristics: Adherent

Culture media: DMEM-H+10%FBS+1%Glutamax+1% Sodium Pyruvate+1%P/S

Passage ratio: 1:3-1:6, change the media every 2-3 days.

Passage period: 48-72 h

Culture conditions: 95% air + 5% CO₂ , 37℃

Freezing conditions: 60% basal medium+30% FBS+10% DMSO, liquid nitrogen storage

Quality test: bacteria, fungi and mycoplasma negative