Cell Name: Stable-overexpressing human KRAS in NCI-H358 cell line
Catalogue No.: CGSC-M26541

Modified Gene: hKRAS

Gene ID: 3845

Modification Type: Overexpression

Method: Piggy-BAC

Transcript: NM_004985.5

Resistance gene: Puro

Fluorescent gene: EGFP

Clone Type: Poolcells

Cellular Identification: PCR, Sequencing

Genus: Homo sapiens

Tissue origin: Lung

Cell morphology: Epithelial cells

Characteristics: Adherent

Culture media: RPMI-1640 ＋10% FBS＋1% P/S

Passage ratio: 1:3-1:6, change the media every 2-3 days.

Passage period: 24-48 h

Culture conditions: 95% air + 5% CO₂ , 37℃

Freezing conditions: 60% basal medium+30% FBS+10% DMSO, liquid nitrogen storage

Quality test: bacteria, fungi and mycoplasma negative