Cell Name: Stable-overexpressing human FDCSP in MDA-MB-231 cell line
Catalogue No.: CGSC-M26471
Modified Gene: hFDCSP
Gene ID: 260436
Modification Type: Overexpression
Method: Piggy-BAC
Transcript: NM_152997.4
Resistance gene: Puro
Fluorescent gene: EGFP
Clone Type: Poolcells
Cellular Identification: PCR, Sequencing
Genus: homo sapiens
Cell morphology: Epithelial cells
Characteristics: Adherent
Culture media: DMEM-H+10%FBS+1%P/S
Passage ratio: 1:3-1:6, change the media every 2-3 days.
Passage period: 48-72h
Culture conditions: 95% air + 5% CO2 , 37℃
Freezing conditions: 60% basal medium+30% FBS+10% DMSO, liquid nitrogen storage
Quality test: bacteria, fungi and mycoplasma negative