HyCyte cell line: HyCyte NK-92 stable cell line for hCD64 overexpression

Catalogue No.: CGSC-M25931

Gene: hCD64

Gene ID: 2209

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：2.0

Target transcript: NM_000566.4

Modification type: Single Clone

Fluorescent gene: —

Resistance gene: Puro

Parental：NK-92

Species：Human

Passage：5

Doubling time：35-40 h

Growth Properties: Suspension

Recommended culture media: Minimum Essential Medium α(MEM α)supplemented with 12.5 % FBS, 12.5 % HS, 0.2 mM Inositol, 0.05 mM β-mercaptoethanol, 0.02 mM Folic Acid, 100 U/mL Recombinant IL-2, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G292).

Subculture: Cells are cultured as a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. The cell culture density is 5×10⁵ to 1×10⁶ cells per mL.

Freezing media: MEMα + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201)